TABLE 1.
Plasmids used in this study
| Plasmid | Promoter | Secretory signal | Antigen |
|---|---|---|---|
| pQE_null | |||
| pSopE2_SopE2_rbdB | sopE2 | SopE2 | TcdB1821–2366 |
| pSseJ_SseJ_rbdB | sseJ | SseJ | TcdB1821–2366 |
| pSptP_SptP_rbdB | sptP | SptP | TcdB1821–2366 |
| pSspH1_SspH1_rbdB | sspH1 | SspH1 | TcdB1821–2366 |
| pSspH2_SspH2_rbdB | sspH2 | SspH2 | TcdB1821–2366 |
| pSteA_SteA_rbdB | steA | SteA | TcdB1821–2366 |
| pSteB_SteB_rbdB | steB | SteB | TcdB1821–2366 |
| ppagC_SspH1_rbdB | pagC | SspH1 | TcdB1821–2366 |
| pSspH2_SspH2_rbdA | sspH2 | SspH2 | TcdA1820–2710 |
| plac_SopE2_rbdA | lac | SopE2 | TcdA1820–2710 |
| plac_SspH1_rbdA | lac | SspH1 | TcdA1820–2710 |
| pnirB_SopE2_rbdA | nirB | SopE2 | TcdA1820–2710 |
| pnirB_SspH1_rbdA | nirB | SspH1 | TcdA1820–2710 |
| ppagC_SopE2_rbdA | pagC | SopE2 | TcdA1820–2710 |
| ppagC_SspH1_rbdA | pagC | SspH1 | TcdA1820–2710 |