TABLE 1.
Compartmentalized cytokine responses in lung, bronchoalveolar lavage fluid, and plasma of WT and Tlr5−/− mice during experimental melioidosisa
| Sample type and cytokine or chemokine | Mean concn of cytokine or chemokine (pg/ml) ± SEM |
|||
|---|---|---|---|---|
|
T = 24 h |
T = 72 h |
|||
| WT | Tlr5−/− | WT | Tlr5−/− | |
| Lung homogenate | ||||
| TNF-α | 1,261 ± 149 | 703 ± 158** | 2,768 ± 501 | 2,769 ± 260 |
| IL-6 | 6,085 ± 1,326 | 6,641 ± 1,464 | 6,054 ± 2,422 | 11,396 ± 2,719 |
| KC | 19,250 ± 2,039 | 13,510 ± 1,105* | 22,613 ± 4,760 | 25,890 ± 4,372 |
| BALF | ||||
| TNF-α | 1,291 ± 319 | 2,178 ± 652 | 3,449 ± 1,422 | 6,097 ± 1,800 |
| IL-6 | 1,058 ± 140 | 2,722 ± 887 | 16,613 ± 2,971 | 11,050 ± 3,233 |
| KC | 4,902 ± 804 | 1,680 ± 217** | 23,284 ± 6,781 | 27,880 ± 7,894 |
| Plasma | ||||
| TNF-α | 7 ± 1 | 8 ± 1 | 104 ± 36 | 365 ± 113 |
| IL-6 | 412 ± 20 | 316 ± 51 | 2,368 ± 820 | 6,054 ± 1,671 |
| IL-10 | BD | BD | BD | BD |
| MCP-1 | 454 ± 38 | 255 ± 42* | 1,221 ± 405 | 5,465 ± 1,557 |
| KC | 11,760 ± 2,754 | 2,608 ± 603** | 16,410 ± 6,111 | 21,086 ± 4,375 |
| IFN-γ | 47 ± 5 | 19 ± 3** | 68 ± 19 | 1,163 ± 452** |
a
Shown are pulmonary, bronchoalveolar lavage fluid (BALF), and systemic cytokine levels after intranasal infection with 5 × 102 CFU wild-type B. pseudomallei 1026b. Wild-type (WT) and Tlr5−/− mice were sacrificed 24 or 72 h after infection. Data are means ± SEM for seven or eight mice per group per time point. TNF-α, tumor necrosis factor alpha; IL, interleukin; KC, keratinocyte chemoattractant; IFN-γ, interferon gamma; BD, below the detection limit. **, P < 0.01; *, P < 0.05 (versus the WT) (Mann-Whitney U test).