FIG 4.

Delivery of PEPCK IDM2 or PB induces systemic immune responses in the spleen. (A) Immunization schedule for DNA vaccination. C57BL/6 mice were immunized twice, 2 weeks apart (n = 4 to 5 per group), with IDM2 or PB. Spleens were harvested 10 days after the final vaccination to analyze antigen-specific T cell responses. (B) The frequency of PEPCK-specific IFN-γ responses (spot-forming units per million splenocytes) induced after vaccination was determined by an IFN-γ ELISpot assay in response to pooled PEPCK peptides.