FIG 6.

Delivery of PEPCK DNA to intradermal sites induces immune responses at the site of vaccination and contralateral sites. C57BL/6 mice were immunized twice, at a 2-week interval (n = 4 to 5 per group), by either i.m. or i.d. EP. Flank or TA skin from vaccination and contralateral sites was harvested 10 days after the final vaccination and analyzed for CD4⁺ T cell responses. (A) PEPCK-specific responses to each plasmid at the site of vaccination determined by intracellular cytokine staining after peptide stimulation. (B) PEPCK-specific CD4⁺ T cell responses to each plasmid at the contralateral site determined by intracellular staining after peptide stimulation.