FIG 1.

A high-fat diet rich in polyunsaturated fatty acids (HFD-P) increases spleen size. The spleen weight (A), body weight (B), spleen weight normalized to body weight (C), liver weight (D), liver weight normalized to body weight (E), thymus weight (F), and thymus weight normalized to body weight (G) in mice fed LFD, HFD-S, and HFD-P for 4 weeks are shown. Data are for 27 mice per group for panels A, B, and C; 6 mice per group for panels D and E; and 10 mice per group for panels F and G. For panels A and C, data from two different experiments were pooled and the results are shown as the estimated marginal means + SEM. For panels B, D, E, F, and G, the data are shown as the mean + SEM. **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.