FIG 8.

Treatment of HBMEC with pilus-enriched fractions increases the number of plasma membrane-associated CRPs. (A) Quantification of subdiffraction-sized CRPs within the plasma membrane of HBMEC pretreated with different concentrations of the pilus-enriched fraction (PeF) at 5 μg, 2.5 μg, or 2.5 μg or with 50 μM 2-APB or not treated. The superresolution images show a dose-dependent enrichment of sphingolipid nanodomains upon pilus treatment. (B) Representative dSTORM images of HBMEC treated with the indicated concentrations of PeF (as shown in panel A). (C) Images of the corresponding dSTORM insets (red squares) from panel B, illustrating the increase in the number of CRPs induced by the addition of PeF. (D) Conventional diffraction-limited microscopy of the sections shown in panel C. Bars, 5 μm (B) and 500 nm (C and D).