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. 2019 Jul 23;87(8):e00345-19. doi: 10.1128/IAI.00345-19

FIG 1.

FIG 1

Antibody levels after immunization. Mice (n = 33/group) were immunized three times intramuscularly (i.m.) at 2-week intervals with 1 μg PilA, 1 μg PE, or 1 μg PE-PilA adjuvanted with alum or AS01, or mice (n = 20/group) were immunized three times intranasally (i.n.) at 2-week intervals with 6 μg PE, 6 μg PilA, or 6 μg PE-PilA adjuvanted with the heat-labile toxin of Escherichia coli (LT). Sera were collected 14 days after the second and the third i.m. injection (days 28 and 42, respectively) or 14 days after the third i.n. injection (day 42). PE-specific and PilA-specific antibody levels were measured by ELISA.