FIG 2.

Inhibition of vitronectin binding. Mice (n = 33/group) were immunized intramuscularly on days 0, 14, and 28 with 1 μg PE, 1 μg PilA, or 1 μg PE-PilA formulated with alum or AS01. Sera were collected on day 42, and a pool of all sera within each group was made. Twofold dilutions of these serum pools were used to inhibit the binding of vitronectin to PE in microtiter plates. Bound vitronectin was detected by specific antibody. The results are expressed as the dilution of serum able to inhibit 50% of vitronectin binding.