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. 2019 Jul 23;87(8):e00345-19. doi: 10.1128/IAI.00345-19

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Copyright © 2019 Ysebaert et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Inhibition of biofilm formation by anti-rsPilA and anti-PE-PilA antibodies. Adult chinchillas (n = 6) were immunized with rsPilA or the PE-PilA fusion protein, and sera were collected 10 days after the third immunization and pooled. NTHi biofilms of three different strains (86-028NP, 1714, 1128) were allowed to form in the wells of 8-well chambered glass slides. Immunized or naive (control) serum pools were added to the culture medium during formation of the biofilms. After overnight incubation, biofilms were fixed and then viewed by confocal scanning microscopy. Images were rendered as orthogonal projections to show a top-down view (to depict the relative spatial distribution of the biofilm within the x-y planes), as well as a side view (to depict the relative biofilm height within the z plane) (A to I), and analyzed with COMSTAT software to evaluate the biofilm thickness (J) and total biomass (K). Bars, 20 μm. P values are for immune sera compared with the control. **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.