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. 2019 Jul 17;9:251. doi: 10.3389/fcimb.2019.00251

Figure 7.

Figure 7

Detection of GlmU relative activity (A) and relative inhibition of H37Ra growth (B) when treated with TPSA and SAM-dependent methylation of TPSA by Rv0558 protein and Rv0560c protein. Detection of relative growth inhibition of H37Ra/pVV2-Rv0558 and H37Ra/pVV2-Rv0560c strain as well as H37Ra/pVV2 strain as a control when treated with different concentrations of TPSA (C). Differences among the groups were calculated by unpaired two-tailed t-test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 [In (A), the P-values of TPSA + SAM group to TPSA + SAM + Rv0558 group for 1.59 μM, P = 0.0174; 2.65 μM, P = 0.0072; 5.3 μM, P < 0.0001; 15.9 μM, P = 0.0001; 26.5 μM, P = 0.0002; 42.4 μM, P = 0.0001 and 53 μM, P < 0.0001. The P-values of TPSA + SAM group to TPSA + SAM + Rv0560c group for 1.59 μM, P = 0.0006; 2.65 μM, P = 0.0068; 5.3 μM, P < 0.0001; 15.9 μM, P = 0.0001; 26.5 μM, P = 0.0037; 42.4 μM, P = 0.0006 and 53 μM, P < 0.0001. In (B), the P-values of TPSA + SAM group to TPSA + SAM + Rv0558 group for 0, P > 0.9999; 3.125 μg/ml, P = 0.1178; 6.25 μg/ml, P = 0.0776; 12.5 μg/ml, P = 0.0793 and 25 μg/ml, P = 0.035. The P values of TPSA + SAM group to TPSA + SAM + Rv0560c group for 0, P > 0.9999; 3.125 μg/ml, P = 0.0203; 6.25 μg/ml, P = 0.4582; 12.5 μg/ml, P = 0.2466 and 25 μg/ml, P = 0.0999. In (C), the P-values of H37Ra/pVV2 group to H37Ra/pVV2-Rv0558 group for 0, P = 0.9964; 3.125 μg/ml, P = 0.1284; 6.25 μg/ml, P = 0.016; 12.5 μg/ml, P = 0.0082 and 25 μg/ml, P = 0.9994. The P-values of H37Ra/pVV2 group to H37Ra/pVV2-Rv0560c group for 0, P = 0.9985; 3.125 μg/ml, P = 0.1041; 6.25 μg/ml, P = 0.1532; 12.5 μg/ml, P = 0.0113 and 25 μg/ml, P = 0.9995].