Figure 4.

Knockdown of U2AF1 impairs enucleation and leads to the generation of erythroblasts with the abnormal nucleus. A, Representative profiles of enucleation as assessed by Syto‐16 staining on day 15 and day 17 of culture. B, Quantitative analysis of enucleation on indicated days from three independent experiments. C, Representative cytospin images of erythroblasts cultured for 15 d. D Quantitative analysis of abnormally nucleated erythroblasts at day 13, day 15 of culture from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001