Table 1.
List of PAAR and VgrG proteins from Xanthomonadales and putative toxic effectors identified by Bastion6 software.
| Species | T6SS Group | PAAR | Orphan PAAR | VgrG (class) | Orphan VgrG (class) | Bastion6 predicted T6SS effectors |
|
|---|---|---|---|---|---|---|---|
| N | Description1,2 | ||||||
| X. vesicatoria ATCC 35937 | 3∗ | 1 | 0 | 1 (I) | 0 | 4 | Hypothetical |
| X. vasicola pv. vasculorum SAM119 | 3∗∗∗ | 0 | 0 | 1 (I) | 0 | 1 | Hypothetical |
| X. translucens pv. undulosa Xtu 4699 | 3∗∗∗ | 0 | 2 | 1 (I) | 0 | 1 | Hypothetical |
| 4 | 1 | 2 (II) | 12# | Tle1-like, DUF3304 (2), neuraminidase | |||
| 2 orphans | Tox-REase-5 (2) | ||||||
| X. perforans LH3 | 3∗ | 1 | 0 | 1 (I) | 0 | 3 | Acid phosphatase, EEP endo/exonuclease/phosphatase |
| 3∗∗∗ | 0 | 1 (I) | 2 | TIR_2 superfamily | |||
| X. oryzae pv. oryzicola CFBP7342 | 3∗∗∗ | 0 | 3 | 1 (I) and 1 (II) | 1 (I) and 10 (II) | 3 | Hypothetical |
| 4 | 1 | 4 (II) | 19 | Tle1-like (4), DUF3304 (5), hydrolase | |||
| 55 orphans | Tle1-like (2), Tle3-like (2), Tle4-like (2), DUF2875 (5), DUF3304 (4), DUF1800, DUF1501, catalase, peptidase (2), PAAR/Rhs MafB19-deaminase, haemolysin, Tox-REase-5 | ||||||
| X. oryzae pv. oryzicola BLS256 | 3∗∗∗ | 0 | 1 | 2 (I) and 1 (II) | 3 (I) and 5 (II) | 1 | Hypothetical |
| 4 | 1 | 2 (I) and 3 (II) | 14 | Tle1-like (4), DUF3304 (3), hydrolase | |||
| 39 orphans | Tle1-like (2), Tle3-like (4), Tle4-like (3), DUF2875 (5), DUF3304 (5), DUF493, peptidase, transglycosylase, carboxypeptidase, Rhs repeat proteins (2), oxidoreductase | ||||||
| X. oryzae pv. oryzae PXO99A | 3∗∗∗ | 0 | 5 | 1 (I) and 1 (II) | 1 (I) and 5 (II) | 2 | Hypothetical |
| 4 | 1 | 3 (II) | 12 | Tle1-like (2), DUF3304 (2), transglycosylase | |||
| 13 orphans | Tle3-like (5), DUF2875 (4) | ||||||
| X. oryzae pv. oryzae MAFF 311018 | 3∗∗∗ | 0 | 3 | 1 (I) | 2 (I) and 7 (II) | 0 | |
| 4 | 1 | 5 (II) | 14 | Tle1-like (2), DUF3304 (5), DUF2345 | |||
| 51 orphans | Tle1-like (4), DUF3304 (6), Tle3-like (5), DUF2875 (5), murein hydrolase D, muraminidase (2), carboxypeptidase, peptidase, PAAR/Rhs XOO_2897-like deaminase, Tox-REase-5 (2) | ||||||
| X. fragariae Fap21 | 4 | 1 | 0 | 4 (II) | 2 (I) and 4 (II) | 12 | Hypothetical |
| 27 orphans | Tle1-like (2), DUF3304 (6), glycoside hydrolase, Rhs repeat protein, PAAR/DUF4150 Colicin-DNAse | ||||||
| X. citri pv. fuscans | 3∗ | 1 | 0 | 1 (I) | 0 | 4 | Acid phosphatase, EEP endo/exonuclease/phosphatase |
| X. citri pv. citri 306 | 3∗ | 1 | 0 | 1 (I) | 0 | 4 | Acid phosphatase, EEP endo/exonuclease/phosphatase, adhesin |
| X. campestris pv. vesicatoria 85-10 | 3∗ | 1 | 0 | 1 (I) | 0 | 4 | Acid phosphatase, EEP endo/exonuclease/phosphatase |
| 3∗∗∗ | 0 | 1 (I) | 4 | TIR_2 superfamily | |||
| X. axonopodis pv. citrumelo F1 | 3∗ | 1 | 0 | 1 (I) | 0 | 4 | Acid phosphatase, EEP endo/exonuclease/phosphatase |
| 3∗∗∗ | 0 | 1 (I) | 3 | TIR_2 superfamily | |||
| Stenotrophomonas sp. LM091 | 1 | 1 | 0 | 1 (II) | 0 | 5 | Tle3-like (2), DUF4274, DUF2875 |
| Stenotrophomonas sp. KCTC 12332 | 4 | 1 | 2 | 1 (I) and 1 (II) | 1 (II) | 4 | Hypothetical |
| 6 orphans | Tle1-like, Tle4-like, DUF4287 | ||||||
| L. rhizovicinus DSM 16549 | 3∗∗∗ | 0 | 0 | 1 (I) | 0 | 1 | Hypothetical |
| L. gummosus 3.2.11 | 4 | 1 | 2 | 1 (I) | 1 (I) | 4 | Transglycosylase, peptidoglycan-binding protein |
| 7 orphans | DUF4157, peptidase, DUF3244/DUF3218 | ||||||
| L. enzymogenes C3 | 3∗∗∗ | 0 | 3 | 1 (I) | 1 (I) | 0 | |
| 4 | 0 | 3 (II) | 7 | Tle4-like protein, hydrolase, peptidase, phospholipase | |||
| 3 orphans | GTP-binding protein, TPR-repeat protein | ||||||
| D. thyooxidans ATSB10 | 3∗∗ | 1 | 0 | 1 (I) | 0 | 1 | Hypothetical |
| D. japonica A8 | 3∗∗ | 1 | 0 | 1 (I) | 2 (II) | 2 | DUF5636, amidase |
| 7 orphans | Phospholipase, muraminidase, peptidase,TPR repeat proteins (3) | ||||||
#All genes flanking the two VgrGs were analyzed. 1Only proteins with putative toxic domains or DUFs previously associated with T6SS are described. 2Number of proteins with identical domains are indicated in brackets when >1.