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. 2019 Jul 17;7:168. doi: 10.3389/fbioe.2019.00168

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Copyright © 2019 Ahn, Lim, Jusoh, Lee, Park, Kim, Kim and Jeon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reconstitution of vascularized three-dimensional tumor-stromal cell spheroid in HA/fibrin composite in the microfluidic platform. (A) Schematic representation of assay to observe angiogenesis with three-dimensional tumor spheroid in HA/fibrin composite. Confocal images and graph for the number of blood vessel sprouts and sprouting length of blood vessels for (B–D) MKN74 and (E–G) SW620 respectively in HA/fibrin composite with varying HA concentration (0.0, 0.2, and 0.4%) (n = 5–7 chips per condition). (Scale bar: 200 μm).