Figure 2.

Promoter and enhancer reprogramming during lung metastasis of breast cancer. (A) Promoter state transformation from MDA‐MB‐231 to LM2‐4175 cells. The colour in heatmap represents the enrichment percentage of H3K4me3, H3K27ac and H3K27me3. Promoter state of LM2‐4175 was shown in red font, and promoter state of MDA‐MB‐231 was shown in blue font. (B) The average expression fold‐change in genes associated with the different type of promoter state transformation. The horizontal axis represents the promoter state of MDA‐MB‐231 and LM2‐4175, in which states of MDA‐MB‐231 are shown before the hyphen, and states of LM2‐4175 are shown after the hyphen. (C) Promoter state transformation of CD70. The region covered by yellow box represents promoter. The promoter of CD70 transformed from repressive state (in MDA‐MB‐231) to active state (in LM2‐4175). Both H4K4me3 and H3K27ac enrichment were increased, while H3K27me3 enrichment was decreased. CD70 expression was also up‐regulated. (D) Identification of gained and lost enhancers based on enrichment fold change (FC) of H3K27ac. (E) Expression FC of genes associated with gained and lost enhancers. (F) Differentially expressed genes associated with both promoter state transformation and enhancer reprogramming. P indicates promoter, E indicates enhancer, and Exp indicates expression. The first row in heatmap represents promoter states of LM2‐4175, the second row represents promoter states of MDA‐MB‐231, the third row represents enhancer changes and the fourth row represents expression FC