Figure 7.

Function validation of transcription factor LMO4 in lung metastasis of breast cancer. (A) The efficiency of LMO4 knockdown was confirmed by Western blotting assay in LM2‐4175 cells transfected with siLMO4 and vector siRNA. β‐actin was used as a control. (B) The efficiency of LMO4 knockdown was confirmed by qRT‐PCR in LM2‐4175 cells transfected with siLMO4 and vector siRNA. (C) Expression changes of predicted target genes of LMO4 after LMO4 knockdown. Expression levels were analysed by qRT‐PCR. Student's t test was used for statistical comparison (*P < 0.05; **P < 0.01). (D) Expression changes of EMT associated genes after LMO4 knockdown. Expression levels were analysed by qRT‐PCR. Student's t test was used for statistical comparison (*P < 0.05; **P < 0.01; ***P < 0.001). (E) The migration abilities were examined by the Transwell assay in LM2‐4175 cells. Student's t test was used for statistical comparison (***P < 0.001)