Figure 2.

Dehydrocostus lactone (DHE) suppressed the mRNA expression of the osteoclast marker genes including NFATc1, dendritic cell‐specific transmembrane protein (DC‐STAMP), cathepsin K, calcitonin receptor (CTR), tartrate‐resistant acid phosphatase (TRAP), matrix metalloproteinase 9 (MMP‐9), c‐Fos, V‐ATPase a3 and osteoclast‐associated immunoglobulin‐like receptor (OSCAR). Bone marrow‐derived macrophages in the presence of macrophage colony‐stimulating factor (30 ng/mL) and receptor activator of nuclear factor‐κB ligand (100 ng/mL) were treated with increasing concentrations of DHE (0, 0.5, 1, 2 or 4 μmol/L) for 5 d. And mRNA expression of osteoclast marker genes was determined by quantitative polymerase chain reaction. The data were expressed as means ± SD. *P < 0.05, **P < 0.01 versus the control group