Figure 4.

Dehydrocostus lactone (DHE) attenuated osteoclastogenesis by inhibiting the NF‐κB signalling. RAW264.7 cells were pre‐treated with dimethylsulfoxide or 4 μmol/L DHE for 4 h and then stimulated with receptor activator of nuclear factor‐κB ligand (RANKL, 50 ng/mL) for 0, 5, 15, 30 or 60 min. Total cell lysates were analysed by immune blotting using the indicated antibodies. The phosphorylation levels of extracellular signal‐regulated kinase, c‐Jun N‐terminal kinase, p38, IKKα/β, p65 and IκBa were determined after treatment with or without DHE