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. 2019 May 31;23(8):5486–5496. doi: 10.1111/jcmm.14432

Figure 5.

Figure 5

CircMTO1 acts as a binding platform for miRNAs. A, RIP experiments were performed using Ago2 antibody on extracts from LX‐2 cells. Relative level of circMTO1 was expressed as fold enrichment in Ago2 relative to IgG immunoprecipitates by qRT‐PCR. B, Expression of circMTO1 in the cytoplasm and nucleus in LX‐2 cells. C, The luciferase activity of pmirGLO‐circMTO1‐Wt in HEK‐293T cells after co‐transfection with the indicated 21 miRNAs or miR‐NC. D and E, The putative‐binding sites of miR‐17‐5p in circMTO1. Relative luciferase activities of circMTO1‐Wt or circMTO1‐Mut were analysed in HEK‐293T cells after co‐transfection with miR‐17‐5p or miR‐NC. F, Interaction between circMTO1 and miR‐17‐5p was validated by Pull‐down assay. Bio‐miR‐NC is not complementary to circMTO1. G, CircMTO1 expression in cells after miR‐17‐5p transfection and miR‐17‐5p level in circMTO1‐overexpressing cells. H, Relative expression of circMTO1 and miR‐17‐5p. *P < 0.05