Figure 2.

BAG3 overexpression promotes proliferation and migration of HPanSteC cells. (A) HPanSteC cells were infected with lentivirus vectors harbouring BAG3 gene. Western blotting was performed to detect the protein levels of BAG3 and α‐SMA. (B) Control or BAG3‐overexpression HPanSteC cells were seeded on an E plate and real‐time cell indexes were monitored using RTCA. (C) De novo DNA synthesis was analysed using Edu incorporation in control or BAG3‐overexpression HPanSteC cells. (D and E) 3  ×  10⁴ of control or BAG3‐overexpression HPanSteC cells were seeded in serum‐free medium in the upper chamber and migration during 24 hours towards the lower chamber, which contained 10% FBS as a chemoattractant, was evaluated. Migrated cells were counted in 10 randomly chosen high power fields. (F) Control or BAG3‐overexpression HPanSteC cells were seeded on CIM‐plate and real‐time cell indexes were analysed using RTCA. *P < 0.01. Error bars indicate means ± SD