Figure 5.

IL‐6, TGF‐β2 and IGFBP2 are involved in maintenance of HPanSteC activation by BAG3 in an autocrine manner. (A and B) The conditional media from BAG3‐overexpression HPanSteC cells were collected and used to treat HPanSteC in the presence of BSA or the indicated monoclonal antibodies. Transwell migration assay was performed in the above cells. (C and D) HPanSteC cells were incubated with conditional media from BAG3‐overexpression HPanSteC cells in the presence of BSA or recombinant CXCL6 (reCXCL6). Transwell migration assay was performed in the above cells. (E and F) HPanSteC cells were incubated with conditional media from BAG3‐overexpression HPanSteC cells in the presence of BSA or the indicated monoclonal antibodies or reCXCL6. Edu incorporation experiments were performed in the above cells. *P < 0.01. Error bars indicate means ± SD