Table 1.
Primary and secondary antibodies used for immunohistochemistry
| Primary antibodies | Secondary antibodies | ||||
|---|---|---|---|---|---|
| Antibody | Company | Dilution | Antibody | Company | Dilution |
| Anti‐Brn‐3a | Santa Cruz | 1:100 | Donkey anti‐goat Alexa Fluor 488 | Dianova | 1:500 |
| Anti‐cleaved caspase 3 | Sigma‐Aldrich | 1:300 | Donkey anti‐rabbit Alexa Fluor 555 | Invitrogen | 1:500 |
| Anti‐GFAP | Life Span Bioscience | 1:2000 | Goat anti‐chicken Alexa Fluor 488 | Molecular probes | 1:1000 |
| Anti‐glutamine synthetase | Santa Cruz | 1:250 | Rabbit anti‐goat Cy3 | Jackson Immuno research | 1:2000 |
| Anti‐opsin | Millipore | 1:1200 | Donkey anti‐rabbit Alexa Fluor 555 | Invitrogen | 1:500 |
| Anti‐PKCα | Santa Cruz | 1:300 | Goat anti‐mouse Alexa Fluor 488 | Invitrogen | 1:500 |
| Anti‐rhodopsin | Abcam | 1:400 | Goat anti‐mouse Alexa Fluor 488 | Invitrogen | 1:500 |
| Anti‐vimentin | Sigma‐Aldrich | 1:100 | 1. Biotinylated anti‐goat IgG | Vector laboratories | 1:500 |
| 2. Streptavidin Alexa Fluor 555 | Life technologies | 1:1000 | |||