Figure 1.

Effects of acute 9 h fasting, E2 and TMX on immobility time in the FST and locomotor activity in the OPT. FST: A and D; OFT: B, C, E and F. N = 6 per group. Data represented as mean ± SEM were analysed by two‐way (diet × treatment) ANOVA followed by post hoc comparisons for the data presented in A, B and C, or by one‐way ANOVA followed by post hoc analysis for the data presented in D, E and F. *** P < 0.001 compared with the non‐fasting + vehicle group; & P < 0.05 compared with the fasting + vehicle group; ### P < 0.001 compared with the E2 + fasting group. VEH: vehicle; E2: 17 β‐estradiol; TMX: tamoxifen