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. 2019 Jun 26;23(8):5165–5175. doi: 10.1111/jcmm.14385

Figure 6.

Figure 6

Overexpression of FOXO3 and PERP reversed the effects of miR‐629 overexpression on cell proliferation, migration and apoptosis of HPASMCs. (A and B) The expression of FOXO3 mRNA and protein in HPASMCs after being transfected with pcDNA3.1 or pcDNA3.1‐FOXO3 was determined by qRT‐PCR and western blot respectively. (C and D) The expression of PERP mRNA and protein in HPASMCs after being transfected with pcDNA3.1 or pcDNA3.1‐PERP was determined by qRT‐PCR and western blot respectively. (E) Cell viability and (F) cell proliferation of HPASMCs after being co‐transfected with miRNAs and plasmids were determined by CCK‐8 assay and BrdU assay respectively. G, The expression of PCNA mRNA in HPASMCs after being co‐transfected with miRNAs and plasmids was determined by qRT‐PCR. H, Cell migration of HPASMCs after being co‐transfected with miRNAs and plasmids was measured by transwell migration assay. I, Cell migration of HPASMCs after being co‐transfected with miRNAs and plasmids was measured by transwell migration assay. Data are expressed as mean ± standard deviation; n = 3. * P < 0.05, ** P < 0.01 and *** P < 0.001 versus control groups