Figure 2.

The canonical Wnt signalling pathway is activated. (A&B) GSK‐3β, p‐GSK‐3β (Ser‐9) and total β‐catenin expression levels were measured in BMSCs and MC3T3‐E1 cells. (C‐H) mRNA expression of Wnt/β‐catenin target genes (Axin2, Lef1, Cnx43) was measured by qPCR in both BMSCs and MC3T3 cells, and the results were normalized to the expression of β‐actin. (I&J) BMSCs and MC3T3‐E1 cells were co‐transfected with Super (8X) TOPFlash reporter and a Renilla control reporter. The cells were cultured in osteogenic differentiation medium with different concentrations of Ginkgolide B (GB) for 48 h. The cells were lysed and luciferase activity was measured. (K&L) Nuclear β‐catenin was measured by western blot in both BMSCs and MC3T3‐E1 cells. *P < 0.05