Figure 2.

Effects of anti‐macrophage receptor with collagenous structure (MARCO) and anti‐Toll‐like receptor 4 (TLR4) mAbs on promoting poly‐methyl methacrylate (PMMA) particles phagocytosis and production of pro‐inflammatory cytokines from aged peritoneal macrophages (pMф) in vitro. Representative contour plots (A) and percentage of young F4/80⁺SSC^high (B) and aged F4/80⁺SSC^high (C) pMф exposed to PMMA particles. Altogether 50 000 fluorescent events were collected. Concentrations of migration inhibitory factor (MIF) (D, F) and tumour necrosis factor (TNF)‐α (E, G) detected from PMMA‐stimulated young and aged pMф, respectively. pMф were exposed to PMMA particle at a ratio of 1:300 in the presence or absence of control Ab, anti‐MARCO or anti‐TLR4 Abs for 24 h. No particle stimulated cells were used as a control. All experiments were repeated at least three times in quadruplicate/condition **P < 0.01, ***P < 0.001; ns, not significant