Figure 1.

KLF8 is a potential biomarker for gastric cancer. A, Heatmap of KLF family genes in five paired primary gastric cancer tissues and liver metastasis using RT‐PCR. Results were normalized by Z‐score. Each column represents a specimen denoted on the above and each role represents a gene which is denoted on the right. Red colour indicates up‐regulated genes and green colour indicates down‐regulated genes. P: primary gastric cancer tissue; M: corresponding liver metastasis. KLF8 was significantly up‐regulated in liver metastases than corresponding primary tumours. B, X‐tile analyses of 5 years overall survival were performed using patient data from TCGA to determine the optimal cut‐off values for KLF8 mRNA. The gastric cancer patients were divided into training and validation sets. X‐tile plots of training sets are shown in the left panels, with plots of matched validation sets shown in the smaller inset. The optimal cut‐off values highlighted by the black circles in left panels are shown in histograms of the entire cohort (middle panels), and Kaplan‐Meier plots are displayed in right panels. P values were determined using the cut‐off values defined in training sets and applying them to validation sets. High KLF8 expression was associated with shorter overall survival (P = 0.006)