Figure 5.

GLUT4 expression in gastric cancer. (A) X‐tile analysed indicated that high GLUT4 expression predicted poor survival in gastric cancer (P = 0.045). GLUT4 expression was further analysed in the validation cohort using IHC staining. The results demonstrated that patients with higher GLUT4 expression levels had worse OS (B) and DFS (C) rates than did patients with lower GLUT4 expression levels. (D) A positive relationship between GLUT4 and KLF8 expression was also observed in the validation cohort (P < 0.001). GLUT4 was knocked down in AGS and MGC803 cells, and the knockdown effect was determined by Western blot (E) and RT‐PCR (F) analysis. Silencing GLUT4 markedly inhibited glucose consumption (G), lactate production (H) and ATP production (I) in both AGS and MGC803 cells (P < 0.05). *P < 0.05