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. Author manuscript; available in PMC: 2019 Jul 24.
Published in final edited form as: Sci Transl Med. 2019 Jun 5;11(495):eaav3963. doi: 10.1126/scitranslmed.aav3963

Fig. 3. Human IgG selectively recognizes the early (stage I/II) giRBC surface.

Fig. 3.

(A) Stage-specific expression of the TdTomato (TdTom) reporter in transgenic Pf2004_164/TdTom parasites by flow cytometry. Reporter expression is shown by fluorescence intensity in a time course across stages I to III gametocytes. (B) The Violet+/TdTomato+ gametocyte population detected by flow cytometry can be separated into weak TdTomato+ (stage I/II gametocytes) and strong TdTomato+ (stage II/III) subgroups. (C) Correlation between TdTomato signal and human IgG based on flow cytometry data (Pearson’s correlation, P < 0.0001). (D) Fluorescence microscopy analysis using the same antibody and reporter combination as above. DAPI, 4′,6-diamidino-2-phenylindole. Left: Surface labeling is present on both asexual parasites (arrows) and early gametocytes (arrowheads). Right: High-content image quantification of fluorescence microscopy data based on proportion of the cell surface that is labeled (top) and stratified by intensity (bottom).