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. 2019 Jul 17;10:1633. doi: 10.3389/fmicb.2019.01633

FIGURE 1.

FIGURE 1

VP1 of CVB3 induces cell cycle arrest at G1 phase. (A,B) HeLa cells were transfected with pEGFP-VP1 or pEGFP-C1 for 24 h. The expression of VP1 was determined by fluorescence microscopy (A, lower panel; arrow heads indicate the nuclei). The relative fluorescence intensity of EGFP was quantified (B). The RNA level of VP1 was determined by RT-PCR (C). (D–F) HeLa cells were transfected with pEGFP-VP1 for 24 h. Control cells were transfected with pEGFP-C1. Cell cycle was analyzed by flow cytometry (D). Cell population distributed in each phase of the cell cycle was compared between the cells expressing VP1 and control cells (E,F). n = 3. *P < 0.05, ∗∗∗P < 0.01.