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VP1 of CVB3 induces G1 arrest in cadrdiomyocytes. (A) Cardiomyocytes were isolated from the hearts of neonatal Balb/c mice. Cells were transfected with pEGFP-C1 or pEGFP-VP1 for 24 h and viewed by fluorescence microscope. (B,C) Cell cycle was determined by flow cytometry. (D,E) Cell population distributed in each phase of the cell cycle was compared between the cells expressing VP1 and control cells. n = 3. *P < 0.05.
