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. 2019 Jul 17;10:1633. doi: 10.3389/fmicb.2019.01633

FIGURE 4.

FIGURE 4

Up-regulated Hsp70 in the cells expressing VP1 results in the dysregulation of cyclin E and p27kip1. HeLa cells were transfected with pEGFP-C1 or pEGFP-VP1 for 24 h. (A) The expression of Hsp70 was determined by RNA-sequencing. (B,C) The expression of Hsp70 was verified by Western blotting. (D,E) HeLa cells were transfected with si-Hsp70 for 24 h. Cell lysate was prepared and cyclin E, p27kip1, and Hsp70 were determined by Western blotting. (F) HeLa cells were transfected with pEGFP-C1 or pEGFP-VP1 for 24 h, HSF1 and p(S326)-HSF1 were determined by Western blotting. n = 3. *P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.