Figure 9. Measurement of plasmalogens and their breakdown product in COCl2 exposed mice.
Equal number of male and female mice were sacrificed 24 h post exposure to either air or 20 ppm COCl2 for 10 min. Plasma was subjected to a modified Bligh-Dyer lipid extraction in the presence of lipid class internal standards including 1-0-tetradecanoyl-sn-glycero-3-phosphoethanolamine and 1,2-ditetradecanoyl-sn-glycero-3-phosphoethanolamine. Lipid extracts were diluted in methanol/chloroform (1/1, v/v) and molecular species were quantified using electrospray ionization mass spectrometry on a triple quadrupole instrument employing shotgun lipidomics methodologies (see Material and Methods for more details). Plasmenylethanolamine, (A) and Polyunsaturated Lysophosphatidylethanolamine (B) levels were measured. Values are means ± 1 SEM. Statistical Analysis by the Student’s t-test