Figure 1.

Angiopoietin‐like protein 8 (ANGPTL8) expression in human NP tissues. (A), Representative histological images of different Pfirrmann degrees NP tissues in HE and Alcian blue staining. Magnification: 400×. (B), ANGPTL8 mRNA level was measured by qRT‐PCR in different Pfirrmann grades of NP tissues. (C), Correlation between ANGPTL8 mRNA level and Pfirrmann grades of NP tissues (n = 40) analysed by non‐parametric linear regression. (D), Representative images and the quantitative statistical analysis of ANGPTL8 protein expression according to the Western blot analysis. GAPDH was used as an internal control. (E,F), Representative images of ANGPTL8 expression and statistical analysis of positive ANGPTL8 cells as detected by immunofluorescence analysis. Magnification: 200×. Data were presented as the mean ± SD (n = 3). *P < 0.05