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. Author manuscript; available in PMC: 2020 Jun 13.
Published in final edited form as: Lipids. 2019 Jun 13;54(6-7):411–418. doi: 10.1002/lipd.12171

Fig. 3.

Fig. 3

MALDI-IMS images of kidney sections from 28-day-old mice show the distribution and relative abudnace of ions with m/z 888.5561, 888.5709, and 888.6267. The abundance of each is indicated by a heatmap. Based on accurate mass, the peaks at m/z 888.5561 and 888.5709 correspond to C13 isotopes of phospholipids (PI(36:4) and PI(36:3), while m/z 888.6267 corresponds to the species of interest, i.e. SulfoHexCer(18:2/24:0). In contrast to the sulfatide, the two phospholipids were present at similar levels in the wild-type and Alport kidneys of 28-day old mice. High mass accuracy is necessary to separate near isobaric lipid species.