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. Author manuscript; available in PMC: 2019 Jul 24.
Published in final edited form as: Cell Rep. 2019 Jul 9;28(2):368–381.e6. doi: 10.1016/j.celrep.2019.06.025

Figure 1. Estrogen Deficiency Disrupts Maintenance of Satellite Cells in Skeletal Muscles of Females.

Figure 1.

(A) Total number of satellite cells quantified by lineage negative;VCAM, alpha7 double-positive cells in five discrete muscles from control (n = 15) and ovariectomized (Ovx; n = 15) mice. Muscles were harvested and analyzed 2, 4, or 7 months after Ovx and in age-matched controls.

(B) Muscle masses.

(C) Total number of satellite cells normalized to muscle masses.

(D) Satellite cells quantified by immunohistochemistry of Pax7+ cells in TA muscles from control (n = 4) and Ovx (n = 4) mice at 2 months of hormone deficiency. Arrows indicate localization of DAPI+ Pax7+double-positive cell. Scale bars, 50 μm.

(E) TA muscle cross-sectional area and number of fibers from control and Ovx mice (p ≥ 0.193), and Pax7+ cells per TA muscle cross-sectional area and % Pax7+ cells in each cross section relative to the total number of muscle fibers.

Significant main effects of two-way ANOVA (p < 0.05) are indicated above the bars (A–C) and when interactions occurred (p < 0.05), Holm-Sidak post hoc tests are indicated by the following symbols: *, different than control at corresponding duration (A–C); ***ø, different than 2- and 4-month Ovx (A and C); and +, different than 2- and 7-month Ovx (B). **p < 0.005 by Student’s t tests (E).