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. Author manuscript; available in PMC: 2019 Jul 24.
Published in final edited form as: Cell Rep. 2019 Jul 9;28(2):368–381.e6. doi: 10.1016/j.celrep.2019.06.025

Figure 5. Estrogen Receptor Alpha (ERα) Is the Relevant Hormone Receptor in Satellite Cells.

Figure 5.

(A) mRNA gene expression of Pax7 in isolated ZsGreen+ satellite cells from Pax7-ZsGreen female mice (n = 4).

(B) Transcripts per kilobase million (TPM) of ER alpha (Esr1), ER beta (Esr2), g-protein coupled ER (Gper1), and progesterone receptor (Pgr) in isolated ZsGreen+ satellite cells from Pax7-ZsGreen mice (n = 3).

(C) mRNA gene expression of Esr1 ZsGreen– -mononuclear cells and Zsgreen+ satellite cells from Pax7-ZsGreen mice (n = 4)

(D) TPM of Esr1, Esr2, Gper1, and Pgr in Pax7+/+;Esr1fl/fl;Pax7-ZsGreen (scERαWT; n = 3) and Pax7CreERT2/+;Esr1fl/fl;Pax7-ZsGreen (scERαKO; n = 3) mice.(E and F) mRNA gene expression of Esr1 and Gper1 (E) relative to Esr1 expression and (F) relative to that in scERαWT in isolated ZsGreen+ satellite cells from scERαWT (n = 3) and scERαKO (n = 4) mice.

ANOVA, Holm-Sidak post hoc tests indicated by **p < 0.005, significantly different from Esr1 (B) or scERαWT (D–F)