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. Author manuscript; available in PMC: 2019 Jul 24.
Published in final edited form as: Cell Rep. 2019 Jul 9;28(2):368–381.e6. doi: 10.1016/j.celrep.2019.06.025

Figure 6. Estradiol Regulates Satellite Cell Number Cell Autonomously through ERα.

Figure 6.

(A) Total number of ZsGreen+ satellite cells in five muscles of Pax7+/+;Esr1fl/fl;Pax7-ZsGreen (scERαWT; n = 6) and Pax7CreERT2/+;Esr1fl/fl;Pax7-ZsGreen (scERαKO; n = 6) mice.

(B) Muscle masses.

(C) Total number of ZsGreen+ cells normalized to muscle mass.

(D) Total number of satellite cells quantified by lineage negative;VCAM,alpha7 double-positive cells in two muscles of scERαWT (n = 6), scERαKO (n = 4), scERαKO+Ovx (n = 5), and scERαKO+Ovx+17β-estradiol (scERαKO+Ovx+E2; n = 5).

(E) Muscle masses.

(F) Total number of satellite cells normalized to muscle masses.

(G) Total number of satellite cells isolated from TA muscles of Ovx mice without (n = 6) and with Bazedoxifine (Ovx+BZA; n = 5) treatment (p = 0.055).

(H) scERαWT and scERαKO transplantation scheme.

(I) Quantification of ZsGreen+ donor satellite cells in control recipient TA muscles following transplantation.

*p < 0.05 by Student’s t tests (A–C, G, and I) *p < 0.05 ANOVA, Holm-Sidak post hoc tests are indicated by *, different from scERαWT (D–F)

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