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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Gene Expr Patterns. 2019 Mar 30;32:53–66. doi: 10.1016/j.gep.2019.03.001

Fig. 1.

Fig. 1.

Immunohistochemical survey of L-plastin expression in early zebrafish development.

A. Enveloping layer (EVL) expression at 50% epiboly, seen in equatorial view.

B. EVL expression at 50% epiboly, seen in cutaway view of a single focal plane. The squamous EVL is strongly stained, in contrast to the deep cells (DC) and the yolk syncytial layer (YSL).

C. EVL expression at 90% epiboly, seen obliquely from the vegetal pole.

D. Periderm expression at the somite stage. Stain intensity is variegated, appearing brighter or darker in adjacent cells.

E. 70% epiboly, intermediate magnification of animal cap EVL.

F. 70% epiboly, high magnification of animal cap EVL. In this single focal plane, L-plastin expression appears as tightly packed cytoplasmic dots or clumps.

DC = deep cells; EVL = enveloping layer; YSL = yolk syncytial layer.