Immunohistochemical survey of L-plastin expression in early zebrafish
development.
A. Enveloping layer (EVL) expression at 50% epiboly, seen
in equatorial view.
B. EVL expression at 50% epiboly, seen in cutaway view of a
single focal plane. The squamous EVL is strongly stained, in contrast to the
deep cells (DC) and the yolk syncytial layer (YSL).
C. EVL expression at 90% epiboly, seen obliquely from the
vegetal pole.
D. Periderm expression at the somite stage. Stain intensity
is variegated, appearing brighter or darker in adjacent cells.
E. 70% epiboly, intermediate magnification of animal cap
EVL.
F. 70% epiboly, high magnification of animal cap EVL. In
this single focal plane, L-plastin expression appears as tightly packed
cytoplasmic dots or clumps.
DC = deep cells; EVL = enveloping layer;
YSL = yolk syncytial layer.