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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Gene Expr Patterns. 2019 Mar 30;32:53–66. doi: 10.1016/j.gep.2019.03.001

Fig. 3.

Fig. 3.

Transgenic expression in lcp1:EGFP-CAAX embryos.

A. 30% epiboly, transgenic embryo in equatorial view. The EVL shows weak, variegated EGFP expression.

B. 70% epiboly, transgenic embryo in equatorial view. Signal intensifies as the EVL spreads over the yolk.

C. 70% epiboly, cutaway view of a single focal plane. Only EVL cells are EGFP +, in contrast to the deep cells (DC) and yolk syncytial layer (YSL).

D. Live embryo at the 15-somite stage. lcp1:EGFP-CAAX labels the periderm, the outermost epithelial layer.

E. Magnification of the tailbud in D, showing variegated EGFP in adjacent peridermal cells.

F. Fixed embryo at 1 dpf, with phal-loidin-568 counterstain. A patch of label-retaining peridermal cells (green) lies over the developing trunk musculature (red).