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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Gene Expr Patterns. 2019 Mar 30;32:53–66. doi: 10.1016/j.gep.2019.03.001

Fig. 9.

Fig. 9.

In vivo validation of zebrafish first intron promoter and enhancer activity.

A. LEFT: 5’ deletion series of the zebrafish 5’ homology arm upstream of the transgenic cassette (lcp1:Cre-P2A-EGFP-CAAX, abbreviated ‘EGFP’). Constructs are numbered according to the number of bases upstream of the engineered translation start site (ATG, where A = +1.) A. RIGHT: Results of deletion-series injections. Green bars = proportion of injected embryos with EGFP + EVL cells. Gray lines = 95% confidence interval on the proportion.

B-F. Animal cap views of injected, EGFP + embryos collected at 50% epiboly.

G. Annotation of the minimal intronic region (−481 to −169) sufficient for EVL activity. The deletion-construct forward PCR primers are underlined. The predicted transcript is in UPPER CASE. TATA = TATA box motif; TSS = transcription start site; DPE = downstream promoter element. The EST ‘foothill’ (See Figs. 7 and 8) is shaded yellow.