Table 1.

Key oligos and primers for the knock-in construct.

guide RNA targeting zebrafish lcp1 exonl
LPLASTIN CRISPR E1 T7	5’-TAA TAC GAC TCA CTA TAG GAG ATG GAG GAG CTC AGA GGT TTT AGA GCT AGA AAT AGC- 3’
lcp1 exonl genotyping primer, ~195 bp fragment
L-plastin CRISPR_E1_T7 geno FWD	5’-TCA ACG TGC TCT GAC TCT GG-3’
L-plastin CRISPR_E1_T7 geno REV	5’-TGC GCA CAT GTC TCT GTA CT-3’
Gibson assembly primers for lcp1 (Cre-P2A-EGFP-CAAX-SV40polyA)
LCP1_CRE_FWD	5’-CAG GTG ATC ATC ATA GCG GGC AAA CAT GGC GAA TTT ACT GAC GGT AC-3’
CRE_P2A_REV	5’-CAG CTT GCT TCA GTA GGC TGA AGT TAG TAG CTC CGC TTC CAT CGC CAT CTT CCA GCA GGC-3’
P2A_EGFP_FWD	5’-CAG CCT ACT GAA GCA AGC TGG AGA CGT GGA GGA GAA CCC TGG ACC TCT GGT GAG CAA GGG CGA GGA G-3’
EGFP_LPC1_REV	5’-CTG ACC GAC TTT AGT GAA GGC GAG GAT CAT AAT CAG CCA TAC CA-3’
lcp1 genomic region, ~2.8 kB on chromosome 9
LCP1 LARGE F1	5’-AGC TTT CAG ATG ATG CAG ATA TCT CGT G-3’
LCP1 LARGE R1	5’-GAG TAT GAG TGC TGT GTT CCC GAC TG-3’