Fig 1. Nanopore sequencing workflow to rapidly identify bacteria in ambulances.

(A) Samples are acquired from three locations in three vehicles over a three-week period, followed by DNA isolation, 16S rRNA gene amplification and sample preparation. The prepared library is then sequenced using the MinION and analyzed to identify bacterial species present. Taxonomic identification is possible within 24 hours from initial sample acquisition. (B) Bioinformatics analysis pipeline for generating taxonomic data.