Table 1.

Overview of Techniques and Their Uses as Described in this Review

	Sanger Sequencing	FISH	LOH Analysis	Next- Generation Sequencing (DNA)	SNP Array	MLPA	WGS/WES	Nanopore Sequencing	(Nested) MSP	RT-MSP	MS-MLPA	Pyrosequencing	Methylation Profiling
Costs per assay	-	-	-	~	~	-	+	~	-	~	-	~	+
Sensitivity	~	~	~	+	+	~	+	-	+	~	+	+	+
Point mutations (eg, IDH1/2, ATRX, TP53)	•			∆		•	•	ø			•	•
Codeletion 1p/19q		ø	ø	•	∆	•	•				•		•
Other CNVs (eg, EGFR amplification)		•	ø	•	∆	•					•		•
Translocations/fusions (eg, KIAA1549-BRAF)		∆		•
Methylation (eg, MGMT promoter/profile)									•	•	∆	•	∆

Abbreviations: ATRX, α-thalassemia/mental retardation syndrome X-linked; BRAF, v-RAF murine sarcoma viral oncogene homolog B; CNV, copy number variation; EGFR, epidermal growth factor receptor; FISH, fluorescent in situ hybridization; IDH1/2, isocitrate dehydrogenase; LOH, loss of heterozygosity; MGMT, methyl guanine methyl transferase; MLPA, multiplex ligation-dependent probe amplification; MS, methylation specific; PCR, polymerase chain reaction; RT-MSP, reverse-transcriptase–methylation-specific polymerase chain reaction; TP53, tumor protein 53; WES, whole-exome sequencing; WGS, whole-genome sequencing.

LEGEND of symbols used: +: High; ~: Medium; -: Low; Δ: Preferred method; •: Possible alternative; ø: Least recommended.

Terms such as “preferred method,” “possible alternative,” and “least recommended” are based on the knowledge and experience of the authors; other laboratories may have a different point of view.