Fig. 4.

—Phylogenomic analysis of AroA in Clostridium group I. (A) The phylogeny shows that AroA, has significantly diverged in all members of the subgroup 3 (C. tetani; clear blue), subgroup 6 (C. argentinense; yellow) and subgroup 1 (C. sporogenes–C. botulinum B; red) from the rest of the subgroups in cluster I (black). The full tree is provided as supplementary Tree 2, Supplementary Material online. (B) Genome context of AroA homologs. C. autoethanogenum is shown as a typical Group I AroA genome context, whereas divergent homologs show a genome context that includes enzymes from pyrimidine metabolism.