Table 1.

Subgroup Core Genome Analysis

Subgroup a	Number of Strains	Species	Proteins Families in Subgroup Core	Core of Cores b
				Proteins in Core	Accessory Proteins	Unique Proteins c
1	106	botulinum A, B, F, sporogenes	890	205	673	2
2	21	autoethanogenum, carboxidivorans, coskatii, drakei, kluyveri, ljungdahlii, magnum, ragsdalei, scatologenes, tyrobutyricum	1,071	205	755	99
3	10	tetani	2,214	205	1,115	877
4	16	acetobutylicum, akagii, arbusti, aurantibutyricum, felsineum, pasteurianum, roseum	1,063	205	752	96
5	42	botulinum C and D, haemolyticum, novyi	1,367	205	824	324
6	11	argentinense, collagenovorans, estertheticum, proteolyticum, senegalense, sulfidigenes, tepidiprofundi, tunisiense	462	205	245	4
7	48	beijerinckii, butyricum(Clostridium type strain), puniceum, saccharobutylicum, saccharoperbutylacetonicum	1,319	205	925	178
8	20	botulinum E	994	205	769	10
9	4	paraputrificum	2,704	205	1,452	1,021
10	8	baratii, colicanis	1,350	205	1,037	94
11	15	chauvoei, disporicum, sartagoforme, saudiense, septicum	1,028	205	779	32
12	55	perfringens	2,044	205	1,276	547
13	3	(Sarcina) ventriculi	2,040	205	1,091	732
14	2	Spp.	1,505	205	1,043	242
15	1	fallax	2,415	205	1,336	854
16	3	cavendishii, intestinale	1,500	205	1,104	178
17	5	algidicarnis, cadaveris, hydrogeniformans	992	205	719	58

^aSubgroups within cluster I are defined by the species tree presented in figure 1.

^bCalculated using representative proteins for each protein family in the core of the subgroups at an identity cut-off of 0.4.

^cThis category includes protein families that are only found in the core of a given subgroup, therefore, they represent subgroup-specific protein families.