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. Author manuscript; available in PMC: 2019 Jul 24.
Published in final edited form as: Nano Res. 2019 May 29;12(6):1473–1481. doi: 10.1007/s12274-019-2418-4

Figure 4.

Figure 4

Specific enrichment of phosphoproteins from a complex human cardiac tissue homogenate. SDS PAGE analysis with (a) SYPRO Ruby and (b) Pro-Q Diamond staining of endogenous phosphoproteins from a HEPES buffer extraction of a human dilated cardiomyopathic (DCM) cardiac tissue homogenate. Equal amount (10 μg) of the loading mixture (LM), flow-through (FT), elution mixture after enrichment (EM), and elution mixture post enrichment with additional dephosphorylation step using alkaline phosphatase (dpEM) was loaded on the gel. The added alkaline phosphatase is a 54 kDa protein denoted by an asterisk (“*”).