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. Author manuscript; available in PMC: 2019 Jul 24.
Published in final edited form as: Nano Res. 2019 May 29;12(6):1473–1481. doi: 10.1007/s12274-019-2418-4

Scheme 1.

Scheme 1

Schematic illustration of the large-scale reproducible synthesis of surface functionalized Fe3O4 NPs as an enabling nanoproteomics platform. (a) Synthesis of starting Fe3O4-OA NPs via thermal decomposition of iron-oleate in the presence of stabilizing ligand, oleic acid (OA). (b) Silane ligand exchange of the hydrophobic Fe3O4-OA NPs to impart a hydrophilic, biocompatible coating. (c) Affinity ligand coupling to free amines imparted by the silanization step enables various downstream biological applications. Specifically, the coupling of a phosphate binding GAPT ligand is highlighted for the current study. (d) Illustration of the key details of the optimized silanization chemistry allowing for robust and reproducible large-scale synthesis of surface functionalized NPs for biological applications.