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. 2019 Jun 11;160(8):1964–1981. doi: 10.1210/en.2019-00013

Figure 2.

Figure 2.

miR-130b-3p expression is decreased, and DENND1A.V2 expression is increased in PCOS theca cells. (A) To validate the decreases in expression of miR-130b-3p, observed from miRNA expression-profiling analysis, miR-130b-3p expression was analyzed by real-time qRT-PCR in theca cells propagated from seven individual normal women and seven individual women with PCOS treated in the absence (c) and presence (f) of 20 μM forskolin for 16 h. miR-130b-3p expression was decreased in PCOS theca cells compared with normal theca cells under basal conditions (**P < 0.01) and forskolin-stimulated conditions (*P < 0.05). (B) The use of mRNA harvested from the identical patients examined above, as we previously reported that DENND1A.V2 expression was increased in PCOS theca cells compared with normal theca cells under both normal (**P < 0.001) and forskolin-stimulated (***P < 0.0001) conditions.