Table 2.
miRNA Target Identification Identified miR-130b-3p as a Possible Candidate Mediating DENND1A.V2 Expression in Normal and PCOS Theca Cells
| ID (hsa) | Targeted Gene |
|---|---|
| miR-130b-3p | DENND1A*** |
| miR-130b-3p | ZNF217 |
| miR-130b-3p | RAB5B |
| miR-130b-3p | LHCGR |
| miR-130b-3p | ERBB3 |
| miR-130b-3p | KCNA4 |
| miR-195-5p | INSR |
| miR-195-5p | YAP1 |
| miR-195-5p | HMGA2 |
| miR-654-5p | GATA4 |
| miR-1271-5p | ERBB3 |
Target identification analyses (miRBase, IPA target filter, and miRNA TargetMiner) predicted that 5 of the 18 differentially expressed miRNAs targeted 9 of the 18 PCOS GWAS loci that have been validated in several ethnic populations. As indicated in boldface, miR-130b-3p was found potentially to target 5 of the PCOS GWAS loci, including DENND1A, ZNF217, RAB5B, LHCGR, and ERBB3. Of particular interest to these studies, miR-130b-3p was observed to target DENND1A***, a functional PCOS GWAS candidate shown to mediate excess androgen production in PCOS theca cells.