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. 2019 Jun 25;160(8):1950–1963. doi: 10.1210/en.2019-00398

Figure 2.

Figure 2.

(A) Demonstration of a full-length Glp2r mRNA transcript in selected mouse tissues. Glp2r and Gapdh mRNA expression was assessed by RT-PCR in total RNA from mouse jejunum, urinary bladder, cerebral cortex, gallbladder, liver, testis, and mesenteric lymph nodes. PCR products (Glp2r 1660 bp, spanning the entire mouse Glp2r ORF, and Gapdh 450 bp) were analyzed by agarose gel electrophoresis using the SYBR Safe DNA Gel Stain. The specificity of each RT reaction (+) was monitored by control reactions using samples in which the RT was omitted from the RT reaction mixture (−). A longer exposure of the gel illustrated in the top blot is shown in the middle blot. Agarose gel images are shown in reverse contrast. (B) Glp2r mRNA expression in jejunum and testis assessed by qPCR using TaqMan gene-expression assays targeting the specified Glp2r transcript exon boundaries. The red filled squares correspond to the expression data using the Glp2r TaqMan assay targeting exons (e)5-6, used to generate the data shown in Fig. 1. Glp2r transcript abundance is reported without endogenous control normalization. Shown are individual data points with overlapping means ± SD (n = 6 mice).